Lipids are extremely diverse class of small biomolecules, for which the full natural variety is yet not fully defined. In addition to the high diversity of original species derived from a classical biosynthesis routes, lipids can undergo various modifications by the introduction of small chemical groups via enzymatic and non-enzymatic reactions, forming another levels of structural complexity, termed “epilipidome”. Lipid oxidation in particular was described for numerous physiological and pathological conditions and generally linked to the regulation of various signaling events. To address diversity of modified lipids and provide functional connection to their signaling role, we are using a range of bioinformatics and analytical solutions based on the application of liquid chromatography coupled online to high resolution mass spectrometry and mass spectrometry imaging to detect, annotate and relatively quantify lipids in various biological samples.
SPP2306 Training School will cover main aspect of mass spectrometry based analysis of lipids and their oxidized forms including:

• Lipid extraction
• Lipid analysis using thin later chromatography couples to mass spectrometry detection
• Design of the liquid chromatography-mass spectrometry methods for detection, identification and relative quantification of lipids, including oxidized ones
• Mass spectrometry imaging of lipids
• Bioinformatics tools for the analysis of LC-MS dat